1. In a sterile PCR tube, set up the following master mix for 6 reactions

1. In a sterile PCR tube, set up the following master mix for 6 reactions (as shown in theshaded column in the table below).Use a new pipette tip for each reagent addition in order to prevent cross contaminationof stock reagents.PCR Reagent FinalConcentrationVolume (in μL)required perreactionVolume (in μL)Required for a 6Reaction MasterMix10X PCR buffer 1X 5 30dNTPs (10 mM stock) 0.16 mM 4 24MgCl2 (25 mM stock) 1.5 mM 3 18Taq DNA polymerase 1U 0.2 1.2Steri …

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