. If the coding region of GFP is cloned behind the lac promoter sequence in the right orientation, there will be expression of GFP in the host bacteria of the plasmid.

The aim of the experiment is to sub clone the coding region fragment of GFP from an ampicillin vector into a Chloramphenicol resistant vector, pBCKS. If the coding region of GFP is cloned behind the lac promoter sequence in the right orientation, there will be expression of GFP in the host bacteria of the plasmid. This will result in bacterial colonies that are Chlor resistant and will exhibit the fluorescent quality of GFP under UV light

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