The yield should be around 200-500 mg\L. the gene product would need to be secreted into the culture medium

The yield should be around 200-500 mg\L. the gene product would need to be secreted into the culture medium . . the functional protein has four disulfide bridges ,the native from is glycosylated but the effect of glycosylation on the function seems not critical . the protein needs proteolytic cleavage for maturation ,An industry -sized facility will built around the product .
Describe the steps to take in order to complete the task including consideration for large scale production.

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